الرئيسية / الفعاليات العلمية / بحوث / The Effect of Interleukin-10 And Its Relationship with The Level of White Blood Cells in Women Spontaneous Miscarriage Undergoing Intracytoplasmic Sperm Injection (ICSI) technique

The Effect of Interleukin-10 And Its Relationship with The Level of White Blood Cells in Women Spontaneous Miscarriage Undergoing Intracytoplasmic Sperm Injection (ICSI) technique

990 Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1
The Effect of Interleukin-10 And Its Relationship with
The Level of White Blood Cells in Women Spontaneous
Miscarriage Undergoing Intracytoplasmic Sperm Injection
(ICSI) technique
Dhirgam F. Hassan Al-Shimerty1; Alaauldeen S. M. AL-Sallami2
1Research Scholar, Faculty of Pharmacy/ University of Kufa; 2Professor , Biology Department/ Faulty of Science/
University of Kufa , Iraq
Abstract
This study was conducted on 84 samples of women subject to the ICSI program . Blood sample was taken to
measure WBC count , Interleukin-10 and β-hCG levels . Groups were divided based on the β-hCG level to
two groups (Pregnant Women Group – Pregnancy Failure Group) and the total of pregnant women divided
into (Pregnancy Group and spontaneous miscarriage) .
The results of the current research showed a significant increase in the level β-hCG and that the increase
in the level of this hormone is evidence of the presence of high success rates for pregnancy in women who
performed operations IVF, where the success rate at the beginning of the matter reached 61.9%, after which
it decreased to 33.3% after the first three months due to the occurrence of spontaneous miscarriage of
pregnant women due to various immunological and physiological reasons, as well as a positive correlation
between the level of β-hCG and other parameters within the study (Interleukin-10 -WBC).
The results of the current research also showed a significant difference between the group (pregnancy
failure) and the group (spontaneous miscarriage) compared with the control group (continued pregnancy)
in relation to the level of Interleukin-10, Also, The results of the current research showed a significant
difference between the group (pregnancy failure) and the group (spontaneous miscarriage) compared with
the control group (continuation of pregnancy) in relation to the level of WBC, and the present study found a
positive relationship between the level of Interleukin-10 and WBC.
Keywords: miscarriage , ICSI technique, Interleukin-10, white blood cells,
Corresponding author
Dhirgam F;
Research Scholar, Faculty of Pharmacy/ University of
Kufa
; E-mail : dhurghamf.hassen@uokufa.edu.iqiq
Introduction
Infertility is a widespread disease worldwide and it
means “the inability of the spouses to achieve pregnancy
within one year of marriage” and the estimated rate of
infertility in the world is around 15-20% (1) .
In vitro fertilization , intracytoplasmic sperm
injection (ICSI) , and intrauterine insemination (IUI) be
are the main methods of assisted reproductive technology
(ART). Found several Various studies in recent years
have indicated that occur risk factor for implantation
failure of women after In vitro fertilization – ICSI which
may be immunological parameters or biochemical
parameters may be affected on In vitro fertilization –
intracytoplasmic sperm injection results (15).
White blood cells (WBCs) They are considered
an important part of the immune system in the body ,It
should be noted that the increase in the number of white
Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 991
blood cells or their lack of a normal limit indicates the
presence of a health problem that afflicts the patient, he
cause of white blood cells rise for a number of reasons,
the most important of which are summarized below For
pregnancy and childbirth or spontaneous miscarriage
because increase Infection in Urinary tract with or
immune system problem (2,16).
Interleukin -10 has several effects and has many
roles in regulating the body’s immunity as well as
affecting inflammation and is involved in stimulating
phagocytic cells as well as has a role in the survival of
B cells and building antibodies, There are many studies
that have demonstrated that interleukin-10a inhibits fatty
polysaccharide and is also stimulated by the bacterial
product of pro-inflammatory (3) .
Materials and Methods
This study was conducted in the laboratories of the
Department of Biology, College of Science, University
of Kufa
, and in the Laboratory of the Fertility Center
in Sadr City Medical City in Najaf Governorate / Najaf
Health Directorate / Ministry of Health / Iraq.
Taked about five milliliters of intravenous blood
samples were drawn in the morning from women
which undergoing intracytoplasmic sperm injection
(ICSI) technique during three parts , the first part after
14 day from injection , the second part after trimester
spontaneous miscarriage while the third part for women
which be continuous pregnant and using a needle and
syringes which is used for one time from each patient
and control. Four milliliters of the blood then it was
left in a gel tube at room temperature for 10 minutes
to complete the blood clotting, then centrifuged at
3000 rpm for 5 minutes, then the serum was separated
for measurements of β-hCG level and Interleukin-10
level by ELISA method and one milliliter of the blood
was put in EDTA-Na2 treated collection tubes for
measurements of number of white blood cells count by
Genex Hematology Analyzer.
Statistical Analysis
The popular statistical system (Graph Pad prism
ver. 5) was adopted, and a one-way analysis of variance
table – Anova method (by Tukey’s multi-comparative
test) was used to compare the groups divided into the
measured parameters. The results are expressed as
(Mean ± Stander Error). Correlation coefficients were
calculated to estimate the correlation between tags
and parameters. Descriptive statistics and correlation
coefficients were performed using mega stat (V10.12
version) for excel 2010 (4).
Results
In this test showed found significant difference
(p<0.05) between pregnant women which was 52
(4.24±0.25 ) and non- pregnant women (Implantation
Failure) (2.56 ±0.10) which was 32 from women which
undergoing intracytoplasmic sperm injection technique
as shown in table (1)
Table (1): Result β- Human chorionic gonadotropin hormone test which differ between pregnant women
which was 52 and non- pregnant women (Implantation Failure) which was 32 from women which
undergoing intracytoplasmic sperm injection technique
P value
hcG (ml U/ml)
Mean ± SEM
Groups Total Number
0.001
32 2.56 ± 0.1
Negative
(Implantation Failure)
52 2.24 ± 0.25
Positive
(Pregnant)
In this test showed found significant difference (p<0.05) between Continuous pregnant women (control
group) which was 28 women and non- pregnant women groups which was 56 from women which undergoing
intracytoplasmic sperm injection technique (Implantation Failure (32) and found non significant difference with
spontaneous miscarriage (24) ) as shown in table (2) .
992 Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1
P value
WBC Count (cell/ml)
Mean ± SEM
Groups Total Number
0.001
Pregnancy Failure) 32 9442 ± 473.8
Spontaneous Miscarriage 24 13860 ± 428.1
Continued Pragnancy 28 7765 ± 331.8
Table (2): Results of White blood cell Count test which differ between Continuous pregnant women (control
group) which was 28 women and non- pregnant women groups which was 56 from women which undergoing
intracytoplasmic sperm injection technique (Implantation Failure (32) and spontaneous miscarriage (24).
In this test showed found significant difference (p<0.05) between Continuous pregnant women (control group)
which was 28 women and non- pregnant women groups which was 56 from women which undergoing intracytoplasmic
sperm injection technique (Implantation Failure (32) and spontaneous miscarriage (24) ) as shown in table (3).
P value
IL-10 (pg/ml)
Mean ± SEM
Groups Total Number
0.001
Pregnancy Failure 32 11.99 ± 0.4
Spontaneous Miscarriage 24 13.83 ± 0.75
Continued Pragnancy 28 6.16 ± 0.72
Table (3) : Results of Interleukin-10 test which differ between Continuous pregnant women (control group) which
was 28 women and non- pregnant women groups which was 56 from women which undergoing intracytoplasmic
sperm injection technique (Implantation Failure (32) and spontaneous miscarriage (24) ).
The study showed the presence of a positive correlation between, Interleukin-10 with White blood cells count
as in table (4).
Table (4): The correlation between Interleukin-10 with WBC Count.
Groups IL-10 P value
WBC Count r = 0.745 0.001
Discussion
The results of the current research showed a
significant increase in the level of significance (p<0.05)
in the level of the β- Human chorionic gonadotropin
hormone, and the reason for this is that this hormone is
evidence of the presence of pregnancy in women who
underwent ICSI operations may be because it is excreted
mainly from the placenta during the formation of the
fetus and this study is consistent with what reached by
some authors (5) in this regard and that the increase in the
level of this hormone is evidence of high success rates of
pregnancy for women who conducted ICSI operations,
where the success rate initially reached 61.9%, after
which it decreased to 33.3% after the first three months
due to the occurrence of spontaneous abortions for
women. Pregnant women for various immunological
and physiological reasons, as this study indicated, and
these results are consistent with what was reached (6-8).
Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 993
The results of the current research showed a
significant increase at the level of significance (p<0.05)
between the group (pregnancy failure) and the group
(spontaneous miscarriage) compared with the control
group (continued pregnancy) in the concentration
of interleukin-10 in When the differences were not
significant between the group (pregnancy failure) and
the group (spontaneous miscarriage may be because
the pregnant women have a low white blood cells level
compared to aborted women because white blood cells
is evidence of infections in the genital and urinary tract
as a result of the spontaneous miscarriage process and
Interleukin-10 is one of the cytokines that stimulates the
occurrence of infections and is responsible for the start of
the inflammatory process and is called the inflammatory
station The fourth of the cells devoured and through
the diagnosis of the concentration of interleukins, the
reaction of the immune system can be known as normal,
high or low, and it is consistent with its findings (9,10) .
As it was explained by increasing the level of
interleukin-10 in aborted women, it is an indication
of the role of interleukins whose function is the
communication between immune cells, whose action is
directed at defending the body. .
The results of the current research showed
a significant increase at the level of significance
(p<0.05) between the group (pregnancy failure) and
the group (spontaneous miscarriage) compared with
the control group (continuing pregnancy) with respect
to white blood cells count while The differences were
significant between the (pregnancy failure) group
and the (spontaneous miscarriage) group, and this
study is consistent with the findings of (11,12) and this
result may be explained that the reason for this is that
pregnant women have a low white blood cells count
level compared to aborted women This may be because
white blood cells count is evidence of the presence of
infections in the genital and urinary tract as a result of
the abortion process, where these results are consistent
with what was reached (11,14) where he explained the
presence of a significant increase in white blood cells
women abortifacients and pregnancy after a period when
the failure of vaccination compared to non- spontaneous
miscarriage women. .
The current study also showed a positive correlation
between the level of interleukin-10 and WBC and
perhaps the reason for this is that the increase in the level
of white blood cells during pregnancy or spontaneous
miscarriage in women, which is often accompanied by
an increase in urinary and genital tract infections which
leads to an increase in the level of interleukin – 10 (15,16) .
Conclusions
The level of interleukin – 10, and increase in the level
of significance (p<0.05) in the level of the β- Human
chorionic gonadotropin hormone and The results of the
current research showed a significant increase at the level
of significance (p<0.05) between the group (pregnancy
failure) and the group (spontaneous miscarriage)
compared with the control group (continuing pregnancy)
with respect to white blood cells count.
Acknowledgements
This work was achieved at Kufa university, faculty
of science
, biology department, and al-Sajjad general
hospital, in addition to workers and all participants. We
would like to express our heartfelt thanks to those who
helped us through this study.
Ethical Clearance : Taken from University of Kufa
ethical committee
Source of Funding : Self
Conflict of Interest : Nil
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